Phospho-IRAK1 (Thr209) Rabbit Polyclonal Antibody

• Catalog Number: orb506111
• Category: Antibodies
• Description: Phospho-IRAK1 (Thr209) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209 RG(p-T)HN
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: Irak1
• UniProt ID: P51617
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: IRAK(phospho T209); p-IRAK(phospho T209); IRAK1(ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-IRAK1 (Thr209)) polyclonal Antibody, Unconjugated (orb506111) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1) Polyclonal Antibody, Unconjugated (orb506111) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRAK1) Polyclonal Antibody, Unconjugated (orb506111) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.