Phospho-Integrin beta 1 (Tyr795) Rabbit Polyclonal Antibody

• Catalog Number: orb184326
• Category: Antibodies
• Description: Phospho-Integrin beta 1 (Tyr795) Rabbit Polyclonal Antibody
• Target: ITGB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Integrin beta 1 around the phosphorylation site of Tyr795 PK(p-Y)EG
• UniProt ID: P05556
• MW: 86 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Integrin beta 1(phospho Y795); p-Integrin beta 1(p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4°C). Primary Antibody: Rabbit Anti-phospho-Integrin beta 1 (Tyr795) antibody (orb184326, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Integrin beta 1 (Tyr795)) Polyclonal Antibody, Unconjugated (orb184326) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.