Phospho-Integrin beta 1 (Tyr783) Rabbit Polyclonal Antibody

SKU: orb186316

Description

Phospho-Integrin beta 1 (Tyr783) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Integrin beta 1 around the phosphorylation site of Tyr783 NPI(p-Y)KS
• Target: ITGB1
• Molecular Weight: 86 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin beta 1(phospho Y783); p-Integrin beta 1(phospho Tyr783); Integrin beta-1; beta 1 subunit; CD 29; CD-29; CD29; CD29 antigen; Fibrinogen Receptor beta subunit; Fibronectin receptor subunit beta; Fibronectin receptor, beta subunit; FNRB; GPIIA; GP IIa; Integrin VLA 4 subunit beta; ITGB; ITGB1; ITGB 1; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; Glycoprotein IIa; Integrin VLA 4 subunit beta; integrin VLA-4 beta subunit; ITB1_HUMAN; very late activation protein, beta polypeptide; VLA BETA; VLA-4 subunit beta; VLA-BETA; VLAB; VLAbeta; Integrin β1.

Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-Integrin beta 1 (Tyr783) antibody (orb186316), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4°C). Primary Antibody: Rabbit Anti-phospho-Integrin beta 1 (Tyr783) antibody (orb186316, Green), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Integrin beta 1 (Tyr783) (orb186316) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Quick Database Links

Gene Symbol

ITGB1

UniProt

P05556