Phospho-Insulin Receptor (Tyr999) Rabbit Polyclonal Antibody

SKU: orb184367

Description

Phospho-Insulin Receptor (Tyr999) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Insulin Receptor around the phosphorylation site of Tyr999 PE(p-Y)LS
• Target: INSR
• Molecular Weight: 70/152 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Insulin Receptor(phospho Y999); p-Insulin Receptor(phospho Y999); CD 220; CD220; CD220 antigen; HHF 5; HHF5; human insulin receptor; INSR; INSR_HUMAN; Insulin receptor subunit beta; IR 1; IR; IR-1; IR1.

Blank control (blue line): HL60 (fixed with 70% ethanol Overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Phospho-Insulin Receptor (Tyr999) antibody (orb184367), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Insulin Receptor (Tyr999)) Polyclonal Antibody, Unconjugated (orb184367) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lovo (Human) cell Lysate at 40 ug, HepG2 (Human) Cell Lysate at 40 ug, Primary: Anti-p-IFNGR1 (Tyr457) (orb184367) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 70kD, Observed band size: 125kD.

Quick Database Links

Gene Symbol

INSR

UniProt

P06213