Phospho-Insulin Receptor Beta (Tyr1355) Rabbit Polyclonal Antibody

• Catalog Number: orb6219
• Category: Antibodies
• Description: Phospho-Insulin Receptor Beta (Tyr1355) Rabbit Polyclonal Antibody
• Target: INSR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Insulin Receptor alpha around the phosphorylation site of Tyr1355 RS(p-Y)EE
• UniProt ID: P06213
• RRID: AB_10918233
• MW: 68/152 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
• Antibody Type: Primary Antibody
• CAS Number: 3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Insulin Receptor(phospho Y1355); Insulin Receptor(
• Note: For research use only

Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-Insulin Receptor Beta (Tyr1355) antibody (orb6219), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 3 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: 293T Cell (Human) Lysate at 30 ug, Primary: Anti-Phospho-Insulin Receptor Beta (Tyr1355) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68/63 kD.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Muscle (Mouse) Lysate at 40 ug, Lane 3: Muscle (Rat) Lysate at 40 ug, Primary: Anti-Phospho-Insulin Receptor Beta (Tyr1355) (orb6219) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kD, Observed band size: 95 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Insulin Receptor Beta (Tyr1355) Polyclonal Antibody, Unconjugated (orb6219) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.