Phospho-INPPL1 (Ser576) Rabbit Polyclonal Antibody

SKU: orb6945

Description

Phospho-INPPL1 (Ser576) Rabbit Polyclonal Antibody

Research Area

Autoimmune, Cell Biology, Growth Factors, Immunology, Lipid Phosphate Phosphatases, Metabolism, Neurogenesis, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SHIP1 around the phosphorylation site of Ser576 QN(p-Y)LD
• Target: INPPL1
• Molecular Weight: 139 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

INPPL1(phospho Ser576); INPPL1(phospho S576); INPPL1(phospho-Ser576); 5-trisphosphate 5-phosphatase 2; 51C protein; EC 3.1.3.n1; inositol polyphosphate phosphatase like 1; Inositol polyphosphate phosphatase like protein 1; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; INPPL1; Phosphatidylinositol 3; Phosphatidylinositol 3, 4, 5 trisphosphate 5 phosphatase 2; Protein 51C; SH2 domain containing inositol 5' phosphatase 2; SH2 domain-containing inositol 5''-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2; SHIP2; SHIP2_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (INPPL1) Polyclonal Antibody, Unconjugated (orb6945) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Uters (Mouse) Lysate at 30 ug, Primary: Anti-p-INPPL1 (Ser576) (orb6945) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 139 kD, Observed band size: 135kD.

Quick Database Links

Gene Symbol

INPPL1

UniProt

O15357