Phospho-IL7R (Tyr449) Rabbit Polyclonal Antibody

• Catalog Number: orb6212
• Category: Antibodies
• Description: Phospho-IL7R (Tyr449) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IL-7Ra around the phosphorylation site of Tyr449 EA(p-Y)VT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: IL7R
• UniProt ID: P16871
• RRID: AB_10934721
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IL7R alpha(phospho Y449); IL-7Rα; IL-7Ra; Interleu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sabrautzki, Sibylle et al. An ENU mutagenesis-derived mouse model with a dominant Jak1 mutation resembling phenotypes of systemic autoimmune disease Am J Pathol, 183, 352-368 (2013)

PubMed: 23791841

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CD127 antibody (orb6212), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: U20s Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-IL-7Ra (Tyr449) (orb6212) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.