Phospho-IGF1R (Tyr1165 + Tyr1166) Rabbit Polyclonal Antibody

• Catalog Number: orb5505
• Category: Antibodies
• Description: Phospho-IGF1R (Tyr1165 + Tyr1166) Rabbit Polyclonal Antibody
• Target: IGF1R
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr1165+Tyr1166 TD(p-Y)(p-Y)RK
• UniProt ID: P08069
• RRID: AB_10952955
• MW: 150 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IGF1 Receptor(phospho Y1165+Y1166); p-IGF1 Recepto
• Note: For research use only

Blank control: Mouse Kidney (blue). Primary Antibody: Rabbit Anti-phospho-IGF1R (Tyr1165 + Tyr1166) antibody (orb5505, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (orb5505, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min on ice. Acquisition of 20000 events was performed.