Phospho-IFNGR1 (Tyr457) Rabbit Polyclonal Antibody

• Catalog Number: orb506146
• Category: Antibodies
• Description: Phospho-IFNGR1 (Tyr457) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human IFNGR1 around the phosphorylation site of Tyr457 EF(p-S)-NH2
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: IFNGR1
• UniProt ID: P15260
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IFNGR1(phospho Y457); p-IFNGR1(phospho Y457); Anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-IFNGR1) Polyclonal Antibody, Unconjugated (orb506146) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: 293T Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-IFNGR1 (Tyr457) (orb506146) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.

Sample: Jurkat (Human) cell Lysate at 40 ug, Primary: Anti-p-IFNGR1 (orb506146) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 53kD, Observed band size: 53kD.

Sample: Panc-1 (Human) Cell Lysate at 40 ug, A431 (Human) Cell Lysate at 40 ug, Primary: Anti-p-IFNGR1 (orb506146) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 53kD, Observed band size: 53kD.