Phospho-IFNAR1 (Ser535+Ser539) Rabbit Polyclonal Antibody

• Catalog Number: orb106014
• Category: Antibodies
• Description: Phospho-IFNAR1 (Ser535+Ser539) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IFNAR1 around the phosphorylation site of Ser535+Ser539 QD(p-S)GNY(p-S)NE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/tets
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: IFNAR1
• UniProt ID: P17181
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IFNAR1(phospho S535+S539); p-IFNAR1(phospho S535+S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-IFNAR1 (Ser535+Ser539) antibody (orb106014), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (INAR1) Polyclonal Antibody, Unconjugated (orb106014) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-IFNAR1 (Ser535+Ser539)) Polyclonal Antibody, Unconjugated (orb106014) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-IFNAR1 (Ser535+Ser539) Antibody (orb106014) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).