Phospho-HSP90 alpha (Thr5 + Thr7) Rabbit Polyclonal Antibody

SKU: orb5489

Description

Phospho-HSP90 alpha (Thr5 + Thr7) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP90 around the phosphorylation site of Thr5 + Thr7 EE(p-T)Q(p-T)QD
• Target: HSP90AA1
• Molecular Weight: 84 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP90AA1 | HSP90 alpha (p-T5/T7); p-HSP90 alpha; phospho-HSP90 alpha; EL52; HEL-S-65p; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp103; Hsp89; Hsp90; LAP-2; LAP2; 86kDa; 89kDa; Hsp86-1; hsp4; HS90A_HUMAN; HSP90AA1; Heat shock 86 kDa (HSP 86 | HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2 | LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; 3.6.4.10; HS90A_MOUSE; Tumor-specific transplantation 86 kDa antigen (TSTA); HS90A_RAT;

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-HSP90 alpha (Thr5 + Thr7)) Polyclonal Antibody, Unconjugated (orb5489) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-HSP90 alpha (Thr5+Thr7) Polyclonal Antibody, Unconjugated (orb5489) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HSP90AA1

UniProt

P07900