Phospho-HSP70 (Tyr41) Rabbit Polyclonal Antibody

SKU: orb5487

Description

Phospho-HSP70 (Tyr41) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr41 PS(p-Y)VA
• Target: HSPA1A
• Molecular Weight: 70 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPA1A | HSP70 (p-Y41); p-HSP70; phospho-HSP70; HSP70-1; HSPA1; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.1; HSP70.2; HSP70I; HSP72; Hsp70-3; Hsp70.3; hsp68; hsp70A1; Hspa1a; Hspa2; HS71A_BOVIN; Heat shock 70 kDa protein 1 (HSP70.1); HS71A_HUMAN; Heat shock 70 kDa protein 1 (HSP70-1 | HSP70.1); Heat shock protein family A member 1A; HSX70; HS71B_HUMAN; HSPA1B; Heat shock 70 kDa protein 2 (HSP70-2 | HSP70.2); Heat shock protein family A member 1B; HS71B_MOUSE; Hcp70.1; HS71A_MOUSE; Heat shock 70 kDa protein 3 (HSP70.3); HSP71_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr41)) Polyclonal Antibody, Unconjugated (orb5487) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Testis (Mouse) Lysate at 40 ug, K562 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-HSP70 (Tyr41) (orb5487) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD.

Quick Database Links

Gene Symbol

HSPA1A

UniProt

P0DMV8