Phospho-HOMER3 (Thr36) Rabbit Polyclonal Antibody

SKU: orb4587

Description

Phospho-HOMER3 (Thr36) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Gallus, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HOMER3 around the phosphorylation site of Thr36 AL(p-T)VS
• Target: HOMER3
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HOMER3(phospho T36); p-HOMER3(phospho T36); HOMER3(phospho T36); HOMER3(phospho Thr36); HOMER 3; Homer 3D protein; Homer homolog 3(Drosophila); Homer neuronal immediate early gene 3; Homer protein homolog 3; Homer3; VESL3; HOME3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HOMER3 (Thr36)) Polyclonal Antibody, Unconjugated (orb4587) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-HOMER3 (Thr36) (orb4587) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.

Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-HOMER3 (Thr36) (orb4587) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.

Quick Database Links

Gene Symbol

HOMER3

UniProt

Q9NSC5