Phospho-Histone H3 (Thr3) Rabbit Polyclonal Antibody

• Catalog Number: orb6170
• Category: Antibodies
• Description: Phospho-Histone H3 (Thr3) Rabbit Polyclonal Antibody
• Target: H3C1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Drosophila, Mouse, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Thr3 AR(p-T)KQ
• UniProt ID: P68431
• RRID: AB_10929595
• MW: 15 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Histone H3(phospho T3); p-Histone H3(phospho T3);
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only

Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Histone H3 (Thr3) antibody (orb6170), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Histone H3 (Thr3) Polyclonal Antibody, Unconjugated (orb6170) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.