Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody

SKU: orb6136

Description

Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody

Research Area

Cell Biology, HDACs

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22 PQ(p-S)PP
• Target: HDAC6
• Molecular Weight: 134 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

HDAC6(phospho S22); p-HDAC6(phospho S22); HDAC6(phospho Ser22); p-HDAC6(Ser22); HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HDAC6 (Ser22)) Polyclonal Antibody, Unconjugated (orb6136) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Jurkat (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-HDAC6 (Ser22) (orb6136) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 134 kD, Observed band size: 134 kD.

Quick Database Links

Gene Symbol

HDAC6

UniProt

Q9UBN7