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Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Catalog Number: orb5754

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5754
CategoryAntibodies
DescriptionPhospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody
TargetHDAC3
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human HDAC3 around the phosphorylation site of Ser424 KE(p-S)DV
UniProt IDO15379
RRIDAB_10933408
MW47 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHDAC3(phospho S424); HDAC3(phospho Ser424); p-HDAC
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NoteFor research use only
Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-Phospho-HDAC3 (Ser424) antibody (orb5754), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Blank control (black line): NIH/3T3. Primary Antibody (green line): Rabbit Anti-Phospho-HDAC3 (Ser424) antibody (orb5754), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-Phospho-HDAC3 (Ser424) (orb5754) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 50 kDa.

Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Sample: MCF-7 (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-HDAC3 (Ser424) (orb5754) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Phospho-HDAC3 (Ser424) Rabbit Polyclonal Antibody

Tissue/Cell: Rat rectum tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-HDAC3, Unconjugated (orb5754) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.