Phospho-GSK3A (Ser21) Rabbit Polyclonal Antibody

SKU: orb14750

Description

Phospho-GSK3A (Ser21) Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cardiovascular Research, Cell Biology, Cell Cycle, Cytoplasmic, Diabetes Research, Epigenetics and Nuclear Signaling, Kinases

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human GSK3 alpha around the phosphorylation site of Ser21 RT(p-S)SF
• Target: GSK3A
• Molecular Weight: 53 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GSK3 alpha(phospho S21); p-GSK3 alpha(phospho S21); GSK3a(phospho-Ser21); P-GSK3 alpha(Ser21); GSK3A_HUMAN; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase 3 alpha; EC:2.7.11.26; Serine/threonine-protein kinase GSK3A; EC:2.7.11.1; GSK 3 alpha; GSK3alpha; GSK-3 alpha; GSK3 alpha; GSK-3alpha; GSK 3A;

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-GSK3 alpha (Ser21)) Polyclonal Antibody, Unconjugated (orb14750) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

GSK3A

UniProt

P49840