Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody

• Catalog Number: orb5359
• Category: Antibodies
• Description: Phospho-GSK3 Beta (Tyr216) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216 VS(p-Y)IC
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: GSK3B
• UniProt ID: P49841
• RRID: AB_10919816
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GSK3 beta(phospho Y216); p-GSK3 beta(phospho Y216)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (phospho-GSK3 Beta (Tyr216)) Polyclonal Antibody, Unconjugated (orb5359) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse NIH/3T3 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Testis tissue lysates, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 50 kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK3 Beta (Tyr216) (orb5359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 49 kD.