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Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody (FITC)
Catalog Number: orb8204
| Catalog Number | orb8204 |
|---|---|
| Category | Antibodies |
| Description | Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody (FITC) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ICC, IF |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Sheep |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21+Ser29 QP(p-S)AFGSMKV(p-S)RD |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | IF=1:100-500, ICC/IF=1:100 |
| Conjugation | FITC |
| Target | GSK3B |
| RRID | AB_10928761 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | GSK3B(Phospho-Ser21+Ser29); GSK3B(Phospho-S21+S29) Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser21+Ser29) antibody (orb8204), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
