Phospho-GRK2 (Ser29) Rabbit Polyclonal Antibody

SKU: orb183982

Description

Phospho-GRK2 (Ser29) Rabbit Polyclonal Antibody

Research Area

Adrenergic Receptors, Cell Biology, G Protein-Coupled Receptors, GPCR Signaling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human GRK2 around the phosphorylation site of Ser29 RA(p-S)KK
• Target: GRK2
• Molecular Weight: 76 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GRK2(phospho S29); p-GRK2(phospho S29); G-protein coupled receptor kinase 2; ADRBK 1; ADRBK1; Adrenergic beta receptor kinase 1; BARK 1; BARK; BARK1; beta adrenergic receptor kinase 1; beta ARK 1; beta ARK1; G Protein Coupled Receptor Kinase 2; G protein dependent receptor kinase 2; FLJ16718; GRK 2; ARBK1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GRK2 (Ser29)) Polyclonal Antibody, Unconjugated (orb183982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

GRK2

UniProt

P25098