Phospho-GNA12 (Ser40) Rabbit Polyclonal Antibody

SKU: orb499645

Description

Phospho-GNA12 (Ser40) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GNA12 RR(p-S)RD
• Target: GNA12
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GNA12(phospho-Ser40); GNA12(phospho Ser40); GNA12(phospho S40); P-GNA12(phospho S40); G alpha 12; gep; GNA12; Guanine nucleotide binding protein alpha 12 subunit; NNX3; RMP; AI414047; AI504261; ALPHA SUBUNIT OF THE G12 FAMILY OF HETEROTRIMERIC GS; FLJ21929; G PROTEIN ALPHA 12; MGC104623; MGC130498; MGC99644; GNA12_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GNA12 (Ser40)) Polyclonal Antibody, Unconjugated (orb499645) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: DU145 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GNA12 (Ser40) (orb499645) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Quick Database Links

Gene Symbol

GNA12

UniProt

Q03113