Phospho-GluR1 (Ser849) Rabbit Polyclonal Antibody

SKU: orb1844

Description

Phospho-GluR1 (Ser849) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser849 QQ(p-S)IN
• Target: GRIA1
• Molecular Weight: 98 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GluR1(phospho Ser849); GluR1(phospho S849); p-GluR1(Ser849); p-GluR1(S849); GLUR 1; GLUR A; AMPA 1; AMPA selective glutamate receptor 1; GLUH 1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Gria 1; Gria1; Gria1; HBGR1; MGC133252.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GluR1 (Ser849)) Polyclonal Antibody, Unconjugated (orb1844) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, U251 (Human) Lysate at 30 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 130 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 128 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, U251 (Human) Lysate at 30 ug, Primary: Anti-phospho-GluR1 (Ser849) (orb1844) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 98 kD, Observed band size: 128 kD.

Quick Database Links

Gene Symbol

GRIA1

UniProt

P42261