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Catalog Number | orb506130 |
---|---|
Category | Antibodies |
Description | Phospho-Gli1 (S112/ T115/ S116) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P |
Predicted Reactivity | Human, Rabbit, Rat |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | KLH conjugated sSynthesised phosphopeptidepeptide derived from human Gli1 around the phosphorylation site of S112/ T115/ S116 IN(p-S)RC(p-T)(p-S)PG |
Antibody Type | Primary Antibody |
Concentration | 1mg/1ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 118 kDa |
Target | GLI1 |
UniProt ID | P08151 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | phospho-Gli1(Ser112/Thr115/Ser116); P-Gli1(Ser112/ Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-Gli1 (T101/S102/S104)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IF | |
Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
APC |
IHC-Fr, IHC-P | |
Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
AP |
IF | |
Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Cy5 |
IF | |
Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Cy3 |
IF | |
Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
FITC |