Phospho-Girdin (Ser1417) Rabbit Polyclonal Antibody

• Catalog Number: orb1726
• Category: Antibodies
• Description: Phospho-Girdin (Ser1417) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human APE around the phosphorylation site of Ser1417 QK(p-S)LT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 206 kDa
• Target: CCDC88A
• UniProt ID: Q3V6T2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Girdin(phospho S1417); p-APE(Ser1417); GRDN_HUMAN;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-APE (Ser1417) antibody (orb1726), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-APE (Ser1417) (orb1726) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 206 kD, Observed band size: 206 kD.