Phospho-GEF H1 (Ser886) Rabbit Polyclonal Antibody

SKU: orb6088

Description

Phospho-GEF H1 (Ser886) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Ras Family, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Arhgef2 around the phosphorylation site of Ser886 RR(p-S)LP
• Target: ARHGEF2
• Molecular Weight: 112 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GEF H1(phospho S886); phospho-Arhgef2(Ser886); Lbcl1; Lfc; mKIAA0651; AA408978; ARHG2; ARHGEF 2; ARHGEF-2; ARHGEF2; GEF; GEF H1; GEF-H1; GEFH1; LFP40; MGC95068; P40; Proliferating cell nucleolar antigen p40; Protein GEF-H1; rho/rac guanine nucleotide exchange factor(GEF) 2; rho/rac guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-GEF H1 (Ser886)) polyclonal Antibody, Unconjugated (orb6088) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control (black line): RAW264.7. Primary Antibody (green line): Rabbit Anti-phospho-GEF H1 (Ser886) antibody (orb6088), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-GEF H1 (Ser886)) Polyclonal Antibody, Unconjugated (orb6088) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Quick Database Links

Gene Symbol

ARHGEF2

UniProt

Q92974