Phospho-GCN2 (Thr899) Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb124639
• Category: Antibodies
• Description: Phospho-GCN2 (Thr899) Rabbit Polyclonal Antibody (PE)
• Target: EIF2AK4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: PE
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 HL(p-T)GM
• Tested applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: GCN2 (phospho T899); p-GCN2 (phospho T899); EIF2AK
• Note: For research use only

Mouse macrophage cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-GCN2 (Thr899) Antibody (orb124639) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (purple), blank (orange).