Phospho-GATA3 (Ser162) Rabbit Polyclonal Antibody

SKU: orb157052

Description

Phospho-GATA3 (Ser162) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human GATA3 around the phosphorylation site of Ser162 DV(p-S)PD
• Target: GATA3
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GATA3(phospho S162); p-GATA3(phospho S162); GATA3(phospho Ser162); p-GATA3(Ser162); GATA 3; GATA3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.

SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-GATA3 (Ser162)) polyclonal Antibody, Unconjugated (orb157052) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

GATA3

UniProt

P23771