Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Rabbit Polyclonal Antibody

SKU: orb6048

Description

Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FOXO1 around the phosphorylation site of Thr24 SC(p-T)WP
• Target: FOXO1
• Molecular Weight: 74 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FOXO4(phospho T32); p-FoxO1(Thr24)/FoxO3a(Thr32); FOXO3A(phospho T32); p-FOXO3A(phospho T32); Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead(Drosophila) homolog 1(rhabdomyosarcoma); Forkhead(Drosophila) homolog 1(rhabdomyosarcoma); Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A; forkhead box protein O3.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (orb6048), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

FOXO1

UniProt

Q12778