Phospho-Fos B (Ser27) Rabbit Polyclonal Antibody

SKU: orb156921

Description

Phospho-Fos B (Ser27) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Fos B around the phosphorylation site of Ser27 QYL(p-S)SV
• Target: FOSB
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AP-1; G0S3; GOS3; GOSB; fra-2; FOSB_HUMAN; FOSB; FosB proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 3; Transcription factor AP-1 subunit FosB; FBJ murine osteosarcoma viral oncogene homolog B; oncogene FOS-B; activator protein 1; FOSB (phospho-S27); p-FOSB; phospho-FOSB; FOSB (phospho-Ser27)

Blank control: Hela (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Polycystin 1 antibody (orb156921), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Fos B (Ser27) (orb156921) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Fos B (Ser27) (orb156921) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Quick Database Links

Gene Symbol

FOSB

UniProt

P53539