Phospho-FLT3 (Tyr591) Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1610099
• Category: Antibodies
• Description: Phospho-FLT3 (Tyr591) Rabbit Polyclonal Antibody (BF647)
• Target: FLT3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human
• Predicted Reactivity: Mouse
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr591 YF(p-Y)VD
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=3ug/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Flt3 / CD135 (phospho Y591); p-CD135 (phospho Y591
• Note: For research use only

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Phospho-FLT3 (Tyr591) antibody (orb1610099), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IGg-AF647, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.