Phospho-FLT3 (Tyr589 + Tyr591) Rabbit Polyclonal Antibody

• Catalog Number: orb5252
• Category: Antibodies
• Description: Phospho-FLT3 (Tyr589 + Tyr591) Rabbit Polyclonal Antibody
• Target: FLT3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr589/591 NE(p-Y)F(p-Y)VD
• UniProt ID: P36888
• RRID: AB_10919740
• MW: 109 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD135 antigen; Fetal liver kinase 2; FL cytokine r
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-FLT3 (Tyr589 + Tyr591) Polyclonal Antibody, Unconjugated (orb5252) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.