Phospho-FGFR1 (Tyr653 + Tyr654) Rabbit Polyclonal Antibody

• Catalog Number: orb5238
• Category: Antibodies
• Description: Phospho-FGFR1 (Tyr653 + Tyr654) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr653/654 ID(p-Y)(p-Y)KK
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 88 kDa
• Target: FGFR1
• UniProt ID: P11362
• RRID: AB_10921723
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FGFR1 alpha(phospho Y654); FGFR1(phospho Y653+Y654
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1 (Tyr653 + Tyr654)) Polyclonal Antibody, Unconjugated (orb5238) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) (orb5238) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 128 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human A673 cell lysates, Lane 4: Human Molt-4 cell lysates, Primary: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) (orb5238) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kDa, Observed band size: 135 kDa.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) (orb5238) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 128 kD.

Tissue/Cell: rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) Polyclonal Antibody, Unconjugated (orb5238) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.