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Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody

SKU: orb183281
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Description

Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human ETS2 around the phosphorylation site of Thr72 LL(p-T)P
TargetETS2
Molecular Weight70 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

ETS2(phospho T72); p-ETS2(phospho T72); ets 2 protein; ETS2; ETS2_HUMAN; ETS2IT1; Oncogene ETS2; Protein C-ets-2; v ets avian erythroblastosis virus E2 oncogene homolog 2; V ets erythroblastosis virus E26 oncogene homolog 2.

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Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ETS2 (Thr72)) Polyclonal Antibody, Unconjugated (orb183281) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody (orb183281)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
DispatchUsually dispatched within 5-10 working days
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