Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody

• Catalog Number: orb183281
• Category: Antibodies
• Description: Phospho-ETS2 (Thr72) Rabbit Polyclonal Antibody
• Target: ETS2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ETS2 around the phosphorylation site of Thr72 LL(p-T)P
• UniProt ID: P15036
• MW: 70 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ETS2(phospho T72); p-ETS2(phospho T72); ets 2 prot
• Research Area: Cardiovascular Research, DNA and RNA, DNA and RNA
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ETS2 (Thr72)) Polyclonal Antibody, Unconjugated (orb183281) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.