Phospho-Estrogen Receptor alpha (Ser104 + Ser106) Rabbit Polyclonal Antibody

• Catalog Number: orb6022
• Category: Antibodies
• Description: Phospho-Estrogen Receptor alpha (Ser104 + Ser106) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Ser104/106 SV(p-S)P(p-S)PL
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1μg /Test, WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 66 kDa
• Target: ESR1
• UniProt ID: P03372
• RRID: AB_10931232
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Estrogen Receptor alpha(phospho S104+S106); p-Estr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): MCF7 (fixed with 80% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Estrogen Receptor alpha (Ser104 + Ser106) antibody (orb6022), dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Estrogen Receptor alpha (Ser104 + Ser106)) polyclonal Antibody, Unconjugated (orb6022) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Estrogen Receptor alpha, p-ESR1) Polyclonal Antibody, Unconjugated (orb6022) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.