Phospho-Estrogen Receptor alpha (Ser305) Rabbit Polyclonal Antibody

SKU: orb5189

Description

Phospho-Estrogen Receptor alpha (Ser305) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Estrogen Receptor alpha around the phosphorylation site of Ser305 KN(p-S)LA
• Target: ESR1
• Molecular Weight: 66 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ESR1 | ER-alpha (p-S305); p-ER-alpha; phospho-ER-alpha; ER; ESR; ESRA; ESTRR; Era; NR3A1; ER-alpha; ERalpha; Estr; Estra; RNESTROR; ESR1_FELCA; ESR1; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1_HORSE; ESR1_HUMAN; ESR1_MOUSE; ESR1_PIG; ESR1_RAT;

Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Phospho-Estrogen Receptor alpha (Ser305) antibody (orb5189), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Quick Database Links

Gene Symbol

ESR1

UniProt

P03372