Phospho-Estrogen Receptor alpha (Tyr537) Rabbit Polyclonal Antibody

• Catalog Number: orb10613
• Category: Antibodies
• Description: Phospho-Estrogen Receptor alpha (Tyr537) Rabbit Polyclonal Antibody
• Target: ESR1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ER alpha around the phosphorylation site of Tyr537 PL(p-Y)DL
• UniProt ID: P03372
• RRID: AB_10746656
• MW: 66 kDa
• Tested applications: FC, ICC, WB
• Dilution range: ICC/IF=1:100, Flow-Cyt=0.2μg /test, WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Estrogen Receptor alpha(phospho Y537); p-Estrogen
• Note: For research use only

Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-Phospho-Estrogen Receptor alpha (Tyr537) antibody (orb10613), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 80% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Estrogen Receptor alpha (Tyr537)) polyclonal Antibody, Unconjugated (orb10613) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.