Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody

SKU: orb156749

Description

Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928 VQ(p-Y)RD
• Target: EPHB1
• Molecular Weight: 106 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EphB1 (p-Y928); p-EphB1; phospho-EphB1; ELK; EPHT2; Hek6; NET; 9330129L11; C130099E04Rik; Cek6; Elkh; Ephb2; Erk; EPHB1_HUMAN; EPHB1; EPH tyrosine kinase 2; EPH-like kinase 6 (EK6 | hEK6); Neuronally-expressed EPH-related tyrosine kinase (NET); Tyrosine-protein kinase receptor EPH-2; 2.7.10.1; EPHB1_MOUSE; EPHB1_RAT; Epth2;

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human U87MG cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kDa, Observed band size: 130 kDa.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 121 kD.

Tissue/Cell: Rat brain, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-EphB1/Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (orb156749) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

EPHB1

UniProt

P54762