Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody

• Catalog Number: orb156749
• Category: Antibodies
• Description: Phospho-Eph receptor B1 (Tyr928) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928 VQ(p-Y)RD
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 106 kDa
• Target: EPHB1
• UniProt ID: P54762
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Eph receptor B1(phospho Y928); EphB1(phospho Y928)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human U87MG cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kDa, Observed band size: 130 kDa.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Eph receptor B1 (Tyr928) (orb156749) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 121 kD.

Tissue/Cell: Rat brain, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-EphB1/Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (orb156749) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.