Phospho-EIF4G1 (Ser1238) Rabbit Polyclonal Antibody

SKU: orb5145

Description

Phospho-EIF4G1 (Ser1238) Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EIF4G1 around the phosphorylation site of Ser1238 AL(p-S)EE
• Target: EIF4G1
• Molecular Weight: 175 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

eIF4G(phospho-Ser1238); eIF4G(phospho-S1238); p-eIF4G(Ser1238); p-eIF4G(S1238); eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4 gamma; EIF4F; EIF4G; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; p220; DKFZp686A1451; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4G-I; IF4G1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EIF4G1 (Ser1238)) Polyclonal Antibody, Unconjugated (orb5145) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EIF4G1

UniProt

Q04637