Phospho-EIF2S1 (Ser51) Rabbit Polyclonal Antibody

• Catalog Number: orb156711
• Category: Antibodies
• Description: Phospho-EIF2S1 (Ser51) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human eIF2 alpha around the phosphorylation site of Ser51 EL(p-S)RR
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: EIF2S1
• UniProt ID: P05198
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: EIF2S1(phospho S51); EIF2S1(phospho-Ser51); EIF2A;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (orb156711) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (orb156711) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-eIF2 alpha (Ser51) (orb156711) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 34 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-eIF2 alpha (Ser51) (orb156711) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 34 kD.