Phospho-eIF2B epsilon (Ser539) Rabbit Polyclonal Antibody

SKU: orb183322

Description

Phospho-eIF2B epsilon (Ser539) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EFHC1 around the phosphorylation site of Ser539 PD(p-S)R
• Target: EIF2B5
• Molecular Weight: 80 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF2B5 | eIF2B epsilon (phospho-S539); p-eIF2B epsilon; phospho-eIF2B epsilon; p-EIF2B5; EIF2B5 | eIF2B epsilon (phospho-Ser539); CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM; VWM5; EI2BE_HUMAN; EIF2B5; eIF2B GDP-GTP exchange factor subunit epsilon; EIF2BE; EI2BE_MOUSE; EI2BE_RAT;

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (orb183322) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (orb183322) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EIF2B5

UniProt

Q13144