Phospho-EGFR (Tyr1068) Rabbit Polyclonal Antibody

SKU: orb10582

Description

Phospho-EGFR (Tyr1068) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Tyr1068 QR(p-Y)SS
• Target: EGFR
• Molecular Weight: 130 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR | ERBB1/HER1 (p-Y1068); p-ERBB1/HER1; phospho-ERBB1/HER1; ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE;

Blank control (blue line): A431 (blue). Primary Antibody (green line): Rabbit Anti-Phospho-EGFR (Tyr1068) antibody (orb10582), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-EGFR (Tyr1068)) polyclonal Antibody, Unconjugated (orb10582) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-EGFR (Tyr1068)) Polyclonal Antibody, Unconjugated (orb10582) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533