Phospho-EGFR (Ser991) Rabbit Polyclonal Antibody

• Catalog Number: orb5994
• Category: Antibodies
• Description: Phospho-EGFR (Ser991) Rabbit Polyclonal Antibody
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser991 LP(p-S)PT
• UniProt ID: P00533
• RRID: AB_10930568
• MW: 130 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Avian erythroblastic leukemia viral(v erb b) oncog
• Research Area: Cancer Research, Cell Biology, Cell Differentiatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): A431 (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-EGFR (Ser991) antibody (orb5994), dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: 293T (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Sample: A431 (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Sample: Hela (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.