Phospho-EGFR (Ser991) Rabbit Polyclonal Antibody

SKU: orb5994

Description

Phospho-EGFR (Ser991) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg /test
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser991 LP(p-S)PT
• Target: EGFR
• Molecular Weight: 130 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR | ERBB1/HER1 (p-S991); p-ERBB1/HER1; phospho-ERBB1/HER1; ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE;

Blank control (blue line): A431 (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-EGFR (Ser991) antibody (orb5994), dilution: 1 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: 293T (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Sample: A431 (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Sample: Hela (Human) Lysate at 30 ug, Primary: Anti-Phospho-EGFR (Ser991) (orb5994) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533