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| Catalog Number | orb5133 |
|---|---|
| Category | Antibodies |
| Description | Phospho-EGFR (Ser1142) Rabbit Polyclonal Antibody |
| Target | EGFR |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Equine, Gallus, Mouse |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser1142 AT(p-S)NN |
| UniProt ID | P00533 |
| RRID | AB_10922426 |
| MW | 170 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Avian erythroblastic leukemia viral(v erb b) oncog Read more... |
| Research Area | Cancer Research, Cell Biology, Cell Differentiatio Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

ICC analysis of Hela cell using phospho-EGFR (Ser1142) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
ICC, IF | |
Equine, Gallus, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Cy5.5 |
ICC, IF | |
Equine, Gallus, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Cy7 |
ICC, IF | |
Equine, Gallus, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
RBITC |
ICC, IF | |
Equine, Gallus, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Cy3 |
ICC, IF | |
Equine, Gallus, Mouse | |
Human | |
Rabbit | |
Polyclonal | |
APC |
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