Phospho-DNA-PKcs (Ser3191) Rabbit Polyclonal Antibody

SKU: orb183273

Description

Phospho-DNA-PKcs (Ser3191) Rabbit Polyclonal Antibody

Research Area

DNA / RNA, DNA Repair, DNA and RNA, Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Canine, Equine, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human DNA PKcs around the phosphorylation site of Ser3191 FL(p-S)KI
• Target: PRKDC
• Molecular Weight: 454 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA PK(phospho S3191); p-DNA PK(phospho S3191); PRKDC(phospho T3191); PRKDC_HUMAN; DNA-dependent protein kinase catalytic subunit; EC:2.7.11.1; PRKDC; HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460;

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-DNA-PKcs (Ser3191)) Polyclonal Antibody, Unconjugated (orb183273) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PRKDC

UniProt

P78527