Phospho-Cytokeratin 17 (Ser44) Rabbit Polyclonal Antibody

SKU: orb5870

Description

Phospho-Cytokeratin 17 (Ser44) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CK 17 around the phosphorylation site of Ser44 LG(P-S)A
• Target: KRT17
• Molecular Weight: 47 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT17 | CK-17 (p-S44); p-CK-17; phospho-CK-17; 39.1; CK-17; K17; PC; PC2; PCHC1; Krt1-17; Ka17; K1C17_HUMAN; KRT17; Cytokeratin-17 (CK-17); Keratin-17 (K17); K1C17_MOUSE; K1C17_RAT; Type I keratin Ka17;

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Cytokeratin 17 (Ser44)) polyclonal Antibody, Unconjugated (orb5870) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human A431 cell lysates, Lane 3: Human U-2 OS cell lysates, Primary: Anti-Phospho-Cytokeratin 17 (Ser44) (orb5870) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 45 kDa.

Quick Database Links

Gene Symbol

KRT17

UniProt

Q04695