Phospho-Cyclin H (Thr315) Rabbit Polyclonal Antibody

• Catalog Number: orb182830
• Category: Antibodies
• Description: Phospho-Cyclin H (Thr315) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Cyclin H around the phosphorylation site of Thr315 EW(p-T)DD
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CCNH
• UniProt ID: P51946
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cyclin H(phospho T315); p-Cyclin H(phospho T315);
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-Cyclin H (Thr315) Polyclonal Antibody, Unconjugated (orb182830) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.