Phospho-CXCR4 (Ser339) Rabbit Polyclonal Antibody

SKU: orb156502

Description

Phospho-CXCR4 (Ser339) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser339 HS(p-S)VS
• Target: CXCR4
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

p-CXCR4(phospho S339); p-CD184(phospho S339); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; CD184 antigen; CXCR4_HUMAN.

Blank control (mouse): Mouse Spleen Cells (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-phospho-CXCR4 (Ser339) antibody (orb156502, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Spleen (Mouse) Lysate at 40 ug, Lane 3: Uterus (Mouse) Lysate at 40 ug, Lane 4: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-phospho-CXCR4 (Ser339) (orb156502) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

CXCR4

UniProt

P61073