Phospho-CREB-1 (Ser121) Rabbit Polyclonal Antibody

• Catalog Number: orb4982
• Category: Antibodies
• Description: Phospho-CREB-1 (Ser121) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser121 TD(p-S)QK
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: CREB1
• UniProt ID: P16220
• RRID: AB_10952027
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CREB(phospho-Ser121); p-CREB-1(Ser121); pCREB-1(Se
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-CREB-1 (Ser121) antibody (orb4982), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Primary: Anti-phospho-CREB-1 (Ser121) (orb4982) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD.