Phospho-CDK1 (Tyr15) Rabbit Polyclonal Antibody

• Catalog Number: orb5823
• Category: Antibodies
• Description: Phospho-CDK1 (Tyr15) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human cdc2 around the phosphorylation site of Tyr15 GT(p-Y)GV
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CDK1
• UniProt ID: P06493
• RRID: AB_10918321
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: phospho-cdc2(Tyr15); CDK1(phospho Tyr15); CDK1(pho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-CDK1 (Tyr15) (orb5823) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 35 kD.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-CDK1 (Tyr15) (orb5823) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 35 kD.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-cdc2 (Tyr15) Polyclonal Antibody, Unconjugated (orb5823) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-cdc2 (Tyr15) Polyclonal Antibody, Unconjugated (orb5823) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.