Phospho-CDK1 (Thr161) Rabbit Polyclonal Antibody

• Catalog Number: orb5824
• Category: Antibodies
• Description: Phospho-CDK1 (Thr161) Rabbit Polyclonal Antibody
• Target: CDK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human cdc2 around the phosphorylation site of Thr161 VY(p-T)HE
• UniProt ID: P06493
• RRID: AB_10921765
• MW: 34 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: phospho-cdc2(Thr161); CDK1(phospho Thr161); CDK1(p
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-CDK1 (Thr161)) Polyclonal Antibody, Unconjugated (orb5824) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-cdc2 (Thr161) Polyclonal Antibody, Unconjugated (orb5824) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.